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“training instructions for epityper quantitative methylation analysis using masscleave for massarray”  (Sequenom)

 
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    Structured Review

    Sequenom “training instructions for epityper quantitative methylation analysis using masscleave for massarray”
    A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. <t>MassARRAY</t> results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).
    “Training Instructions For Epityper Quantitative Methylation Analysis Using Masscleave For Massarray”, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%E2%80%9Ctraining+instructions+for+epityper+quantitative+methylation+analysis+using+masscleave+for+massarray%E2%80%9D/%E2%80%9Ctraining+instructions+for+epityper+quantitative+methylation+analysis+using+masscleave+for+massarray+/pmc05356698-161-23-24
    Average 90 stars, based on 1 article reviews
    “training instructions for epityper quantitative methylation analysis using masscleave for massarray” - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer"

    Article Title: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.12626

    A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. MassARRAY results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).
    Figure Legend Snippet: A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. MassARRAY results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).

    Techniques Used: Methylation Sequencing, Clone Assay, Methylation, Amplification

    Related Articles

    Polymerase Chain Reaction:

    Article Title: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer
    Article Snippet: .. The PCR annealing Tm was 56°C, and sample preparation was performed according to “Training Instructions for EpiTYPER Quantitative Methylation Analysis Using MassCLEAVE for MassARRAY” (Sequenom). ..

    Sample Prep:

    Article Title: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer
    Article Snippet: .. The PCR annealing Tm was 56°C, and sample preparation was performed according to “Training Instructions for EpiTYPER Quantitative Methylation Analysis Using MassCLEAVE for MassARRAY” (Sequenom). ..

    Methylation:

    Article Title: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer
    Article Snippet: .. The PCR annealing Tm was 56°C, and sample preparation was performed according to “Training Instructions for EpiTYPER Quantitative Methylation Analysis Using MassCLEAVE for MassARRAY” (Sequenom). ..



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    Sequenom “training instructions for epityper quantitative methylation analysis using masscleave for massarray”
    A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. <t>MassARRAY</t> results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).
    “Training Instructions For Epityper Quantitative Methylation Analysis Using Masscleave For Massarray”, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%E2%80%9Ctraining+instructions+for+epityper+quantitative+methylation+analysis+using+masscleave+for+massarray%E2%80%9D/%E2%80%9Ctraining+instructions+for+epityper+quantitative+methylation+analysis+using+masscleave+for+massarray+/pmc05356698-161-23-24
    Average 90 stars, based on 1 article reviews
    “training instructions for epityper quantitative methylation analysis using masscleave for massarray” - by Bioz Stars, 2026-09
    90/100 stars
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    A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. MassARRAY results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).

    Journal: Oncotarget

    Article Title: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer

    doi: 10.18632/oncotarget.12626

    Figure Lengend Snippet: A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. MassARRAY results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).

    Article Snippet: The PCR annealing Tm was 56°C, and sample preparation was performed according to “Training Instructions for EpiTYPER Quantitative Methylation Analysis Using MassCLEAVE for MassARRAY” (Sequenom).

    Techniques: Methylation Sequencing, Clone Assay, Methylation, Amplification